Corporate Explore all. About Bio-Rad Back. About Bio-Rad Explore all. Investor Relations Explore all. Description Ordering Accessories. Ordering items Use the filters below to refine results! Pkg of 1, gel polyacrylamide gel casting chamber, includes 8 acrylic blocks, 15 separation sheets, tapered luer connector, stopcock valve. This product is being discontinued soon.
Please contact customer service for more information. Pkg of 1, polyacrylamide gel casting chamber with 0. Pkg of 1, polyacrylamide gel casting chamber with 1.
Discontinued Acrylic Blocks. Discontinued Separation Sheets. Pkg of 5, spacer plate with 0. Pkg of 5, spacer plate with 1. Discontinued Gel Releasers. Since every protein-chemical reagent combination has not been assayed, it is possible that some of the listed reagents produce interference in combination with certain proteins.
However, with respect to proteins such as bovine serum albumin and gamma globulin, the listed reagents show little or no interference.
The acceptable concentrations of reagents for the Standard Procedure are shown in Table 1. Table 1. Since every proteinchemical reagent combination has not been assayed, it is possible that some of the listed reagents produce interference in combination with certain proteins. However, with respect to proteins such as bovine albumin and globulin, the above listed reagents show little or no interference. Protein Assay Dye Reagent Concentrate catalog number contains ml of solution containing dye, phosphoric acid, and methanol.
One bottle of dye reagent concentrate is sufficient for assays using the standard assay procedure, or 2, assays using the microassay procedure.
The Dye Reagent Concentrate can be purchased in a kit with one of two standards: Bovine gamma globulin Kit I, catalog number or bovine serum albumin Kit II, catalog number Cuvettes with 1 cm path length matched to laboratory spectrophotometer. To reconstitute the lyophilized bovine gamma globulin and bovine serum abumin standards, add 20 ml of deionized water and mix until dissolved.
Filter through Whatman 1 filter or equivalent to remove particulates. This diluted reagent may be used for approximately 2 weeks when kept at room temperature. Prepare three to five dilutions of a protein standard, which is representative of the protein solution to be tested.
The linear range of the assay for BSA is 0. See Common Questions, question 4, for more information. Protein solutions are normally assayed in duplicate or triplicate.
Add 5. Incubate at room temperature for at least 5 minutes. Absorbance will increase over time; samples should incubate at room temperature for no more than 1 hour. Measure absorbance at nm. Prepare three to five dilutions of a protein standard which is representative of the protein solution to be tested.
The linear range of the assay for BSA is 1. The linear range of the Standard and Microassay procedures when used in the microtiter plate format is slightly changed, since the ratio of sample to dye is modified. This instrument should not be modified or altered in any way. Alteration of this instrument voids the warranty and safety certification and creates a potential safety hazard. This instrument is intended for laboratory use only.
Bio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is intended, or by modifications of the instrument not performed by Bio-Rad Laboratories or an authorized agent.
Follow the safety specifications listed in this section and throughout this manual. Use only the power cord supplied with the instrument, making sure to choose the plug adaptor that corresponds to the electrical outlets in your region.
Do not block the fan vents. These or other loose items may be pulled into the fan intake, causing damage to the unit due to overheating and voiding the warranty. Operating the cell outside these conditions voids the warranty. No user-serviceable parts are inside the instrument. Contact Bio-Rad service personnel for service. Take appropriate measures to avoid interference. This makes it possible to run different sample types, different gradients, and multiple protocols all at the same time. The i12 focusing trays and electrode assemblies accommodate all possible gel configurations gel-side up or down, with or without electrode wicks.
It also allows sample loading either by inclusion in the rehydration solution in-gel loading or with sample cups sample cup loading. The cell is fully programmable from the user interface; connection to an external computer is not required. Each protocol can contain up to ten steps in which voltage, manner of voltage ramping, current, and duration hr or V-hr are defined. Preprogrammed protocols stored in the internal memory serve as a convenient starting point for developing optimized, sample-specific IEF conditions.
A USB flash drive can also be used as an alternative storage location or method of transfer for protocols and run data files. The application can be used to generate reports, print graphs, and create protocols.
This individual. It also allows programming of different protocols. The touch-screen user interface is used to operate the cell, retrieve preprogrammed and user-defined protocols, create new or edit saved protocols, and access the internal memory for file management. New protocols, sample details, and run data are stored in the internal memory or on an external USB flash drive.
A, Front view with all lids closed; B, Front view showing the safety lid closed and opaque lid open; C, Back view. Table 1. Accessories not included may be purchased separately see Appendix G, Ordering Information. Collect salts and other charged impurities as well as proteins with isoelectric points pI outside the pH range of the IPG strip use is recommended.
For cleaning the focusing tray and electrode assemblies For cleaning the focusing tray and electrode assemblies. Used to manipulate touch screen user interface Indicates whether the IEF cell is level. Channels in the i12 focusing tray Figure 1. Each focusing tray accommodates up to 12 IPG strips. Separate trays are available for 7, 11, 13, 17, 18, and 24 cm IPG strips.
For flexibility, the PROTEAN i12 focusing tray and electrode assemblies accommodate all run configurations: gel-side down or up, with in-gel sample loading or sample cup loading.
Focusing trays can also be used for rehydration of IPG strips. The electrode assemblies Figure 1. The assemblies attach to the focusing tray to provide contact between each IPG strip and its power supply. They accommodate all focusing tray sizes. Carefully inspect the shipping container for any damage that may have occurred during shipping. Severe damage to a container may indicate damage to its contents. If you suspect damage to the contents, immediately file a claim with the carrier in accordance with their instructions before contacting Bio-Rad Laboratories.
Open the shipping carton and lift the content out of its packing. Inspect the instrument for external damage. If any part is missing or damaged, contact Bio-Rad Laboratories immediately. Position the cell so that there is access to the USB ports back panel and power switch right panel.
DO NOT block the fan vents. Make sure the system is on a level surface. Place the leveling bubble in the center of the cooling platform and adjust the instrument leveling feet as needed. Connect the cell to a three-prong, grounded AC outlet using the power cord provided with the cell. Do not place cloth or absorbent pads underneath the instrument. Figure 1.
For more details about performing 2-D electrophoresis, please refer to bulletin , 2-D Electrophoresis for Proteomics: A Methods and Product Manual. Each electrode accommodates the use of electrode wicks and both the gel side-up and gel side-down IEF configurations: a bridge on each electrode fits into the recessed area of the focusing tray to create the flat surface required for the gel-side down configuration, and the electrodes are spring-loaded, which allows them to exert a gentle downward pressure for the gel-side up configuration.
Several features of the electrode assemblies ensure their correct and complete attachment to the focusing tray Figure 2. Square and round orientation pins in the electrode assemblies ensure correct positioning of the cathode and anode in the focusing tray. Grasp the electrode assembly by the release clips and position the orientation pins as shown in Figure 2. Push down on the green tabs until the locks click into place on the walls of the focusing tray.
With the gel-side down configuration, make sure that each electrode is properly seated in the recessed area of the focusing tray.
To remove electrode assemblies from the focusing tray, grasp the green tabs on the release clips and gently squeeze inward. Position the assembly on the Peltier platform. Use the positioning guides and stops on the platform and focusing tray as guides Figure 2.
When the the tray positioning stop reaches the positioning guide on the platform, the assembly is seated correctly Figure 2. Make sure the negative pin on the negative - electrode is in direct contact with the metal gounding strip on the instrument Figure 2.
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